Diploma in Medical Laboratory Technology Syllabus
2 year DMLT + One year Post Diploma Course
SYLLABUS
FIRST YEAR
1) ENGLISH & COMPUTER FUNDAMENTAL
2) ANATOMY & PHYSIOLOGY
3) BIOCHEMISTRY
4) MICROBIOLOGY AND PARASITOLOGY
5) HEMATOLOGY & BLOOD BANKING
6) HISTOTECHNOLOGY - 1
ANATOMY & PHYSIOLOGY
- Introduction to the Human Body
- Short Notes on Surface Anatomy
- The Skeletal System- Bones of Skull and Thoracic Cage
- The Vertebral Colum and Pelvic Girdle
- The Skeleton of the Upper Limb
- The Skeleton of the Lower Limb
- The Joints of the Skeleton
- The Muscles of the Skeleton
- The Circulatory System
- The Blood
- The Principle Blood Vessels
- The Lymphatic System, the Spleen and the Reticulo-Endothelial System
- The Classification of Food
- The Alimentary Canal and the Digestion of Food
- The Liver, Gall- Bladder and Pancreas
- The Respiratory System and Respiration
- Metabolism
- The Endocrine Organs
- The Skin
- The urinary System
- The organs of the Reproductive System
- The Cerebrospinal Nervous System
- The Autonomic Nervous System
- Organs of Special Sense-Taste and Smell
- Organs of Special Sense- Eye and Sight
- Organs of Special Sense- Ear and Hearing
- Glossary of Eponyms
MICROBIOLOGY AND PARASITOLOGY
- General Bacteriology
- Introduction
- History and Scope
- Morphology of Bacteria
- Nutritional Requirement of Bacteria
- Bacterial Metabolism
- Media for Bacterial Growth
- Classification and identification of Bactria
- Sterilization and Disinfection
- Infection
- Antimicrobial Therapy
- Bacterial Genetics
- Immunity
- Antigen
- Antibody
- Antigen and Antibody Reactions
- The Complement System
- Structure and Function of Immune System
- The Immune Response
- Hypersensitivity
Systemic bacteriology:-
- Staphylococcus
- Streptococcus
- Pnuemococcus
- Neisseria
- Cornybacterium
- Bacillus
- Mycobacterium
- Bacillus
- Mycobacterium
- Bacillus
- Clostridium
- Enterobacteriacae
- Pseudomonas
- Vibrio
- Campylobacter
- Heliobacter pyloridis
- Brucella
- Hemophilus
- Spirochaetes
- Rickettsiae
- Chlamydae
- Microbiology in Oral Cavity
Virology:-
- General Characteristics of Viruses
- Chemotherapy of viral Diseases
- RNA Viruses
- DNA Viruses
- AIDS
- Mycology
Parasitology:-
- Introduction
- Protozoa
- Helminthes
BIOCHEMISTRY
- Biochemistry and Medicine
- Biomolecules and Biochemical Methods
- Water and PH
- Structure and Functions of Enzymes and Proteins
- Aminoacids
- Peptides
- Proteins
- Enzymes
- Bioenergetics & the metabolism of carbohydrates and lipids
- Bioenergetics
- Biologic Oxidation
- Oxidation Phosphorylation and mitochondrial Transport Systems
- Carbohydrates of physiologic Significance
- Lipids of Physiologic Significance
- The Citric Acid Cycle
- Gycolysis
- Metabolism of Glycogen
- Gluconeogenesis and control of Blood Glucose
- Fatty Acids
- Biosynthesis of Fatty Acids
- Oxidation of Fatty Acids
- Lipid Transportation and Storage
- Cholesterol
- Metabolism of Protein and amino acids
- Non Essential Amino acids
- Conversion of Amino acids into Specialized Products.
- Structure Function and Replication of Protein
- RNA
- DNA
- Hormones
- Nucleotides
- Nucleic Acids Structure and Function
- Protein Synthesis, Processing and Metabolism
- Membranes: Structure, Assembly and Function
- Vitamins
- Electrolytes
- Glycoprotein's
- Plasmaprotines
- Xenobiotics
ENGLISH & COMPUTER FUNDAMENTAL
Communication:-
- Role of Communication
- Defining Communication
- Classification of Communication
- Purpose of communication
- Major Difficulties in Communication
- Barriers to Communication
- Characteristics of Successful communication – The Seven Cs
- Communication at the Work Place
- Human Needs and Communication "Mind Mapping"
- Information Communication
Comprehension Passage:-
- Reading Purposefully
- Understanding What is Read
- Drawing Conclusion
- Finding and Analysis
Explaining:-
- How to explain Clearly
- Defining and giving reasons
- Explaining Differences
- Explaining Procedures
- Giving Direction
Writing Business Letters:-
- How to contrast correctly
- Formal language
- Address
- Salutation
- Body
- Conclusion
Report Writing:-
- Reporting an accident
- Reporting what happened at a session
- Reporting what happened at a meeting
BASICS OF COMPUTER
Course Content
Introduction to computer – I/O devices – memories- RAM and ROM – Different kinds of ROM- kilobytes. MB, GB their conversion – large computer – Medium, Micro, Mini computers – Different computer language - Numbers system – Binary and decimal conversions – Different operating system – MS DOS – Basic commands – MD< CD, DIR, Type and COPY CON commands – Networking – LAN, WAN, MAN (only basic ideas)
Typing text in MS word – Manipulating text – Formatting the text – using different font sizes, bold, italics – Bullets and numbering – Pictures, file insertion – Aligning the text and justify - Choosing Paper size – adjusting margins – Header and footer, inserting page No's in a document – Printing a file with options – Using spell check and grammar – Find and replace – Mail merge – inserting tables in a document.
Creating table in MS-Excel – Cell editing – Using formulas and functions – Manipulating data with excel – Using sort function to short function to short numbers and alphabets – Drawing graphs and charts using data in excel – Auto formatting – Inserting data from other worksheets.
Preparing new slides using MS-POWERPOINT – Inserting slides – slides transition and animation – Using templates – Different text and font sizes – slides with sounds – Inserting clip arts, pictures, tables and graphs – Presentation using wizards.
Introduction to Internet – Using search engine – Google search – Exploring the next using Internet Explorer and Navigator – Uploading and Download of files and images – E-mail ID creation – Sending messages – Attaching files in E-mail – Introduction to "C" language – Different variables, declaration, usage – writing small programs using functions and sub – functions.
Practical
- Typing a text and aligning the text with different formats using MS-Word.
- Inserting a table with proper alignment and using MS-Word
- Create mail merge document using MS-Word to prepare greetings for 10 friends
- Preparing a slide show with transition, animation and sound effect using MS-PowerPoint
- Customizing the slide show and inserting pictures and tables in the slides using MS-PowerPoint
- Creating a worksheet using MS-Excel with data and sue of functions
- Using MS-Excel prepare a worksheet with text, date time and data
- Preparing a chart and pie diagrams using MS-Excel
- Using Internet for searching, uploading files, downloading files creating e-mail ID
- Using C language writing programs using functions.
BLOOD BANKING & BLOOD TRANSFUSION
Blood banking
Basic principle in blood banking – Organization, planning and documentation. Introduction organization and planning; documentation.
Blood Groups:- Introduction ; ABO blood groups ; principle of ABOI group determination; Technique of blood grouping ; Rh Blood groups, Rh groups and Hemolytic Disease of the new born ; Technique of Rh Grouping.
Selection of donors and collection of blood; - Criteria for selecting donors; collection of blood. The compatibility test, coombs test; platelets, Cryoprecipitate and Fibrinogen BLOOD TRANSFUSION, DONOR SERENING, CROSS MATCHING, BLOOD AND ITS PRODUCTS HISTORICAL ASPECTS OF BLOOD TRANSFUSION, COMPOSITION OF BLOOD, BLOOD GROUP [GENETICS,
Synthesis of blood group antigens, distribution of blood group antigens on cell& fluid. Own laboratory. The result of testing shall be recorded on the label of the container also. Procedure of blood collection & grouping. ABO & Rh grouping Laboratory tests related to blood group abnormalities – comb's test/Ant globulin test. (Direct & Indirect) Tiration of ABO & Rh antibodies. Organization of camps & selection of donors.
Collection of blood from donor (phlebotomy), Autologous blood transfusion, its indication & hazards. Compatibility testing –
- Major cross matching
- Minor cross matching
- Coomb's cross matching
- Cross matching of Universal donors
Blood components –Preparation & storage, aphaeresis, blood & its products. Complication of blood transfusion, Investigation in case of mismatched transfusion. Quality control & maintenance of blood bank safety. Current developments in blood banking. Commercial preparation of synthetic blood. Proficiency test of blood bank personals.
HEMATOLOGY
- Blood –A connective tissue introduction
- Functions of blood
- Formation of blood
- Blood composition
- Plasma
- Blood cells
- Hemoglobin
- Blood coagulation
- Immune- hematology
- Antigens
- Antibodies
- Phagocytosis
- Pinocytosis
- Coagulation mechanism
- Preservation of blood
- Anti coagulants
- Coagulants
- Collection of blood samples
- Blood groups
- ESR, MCHC, PCV, MCH
- CT, BT, PTCC,
- Disorders of blood
- Anaemia
- Leucocytosis
- Thrombocytopenia
- Bleeding disorders
- Clotting disorders
- Thalessemia
- Disorders of white blood cells
HISTOTECHNOLOGY
- Cell
- Tissue
- Microscopes
- Histotechnology equipments and apparatus
- Collection of a tissue
- Fixation and fixatives
- Tissue processing in detail
- Microtome's
- Section cutting
- FNAC
- Biopsy
- Autopsy
- Staining of tissues
- Museum techniques
- Exfoliative cytology
DMLT 2ND YEAR
4. PATHOLOGICAL INVESTIGATION 2
5. HEMATOLOGY AND BLOOD BANKIING 2
6. HISTOTECHNOLOGY 2
LABORATORY MANAGEMENT
- Plan of clinical laboratory
- Work culture of a clinical laboratory
- Professional code of ethics in a laboratory
- Sanitation and hygiene in a laboratory
- Waste management in a laboratory
- Record keeping in a laboratory
- Reception of an laboratory
- Basic infra structure of a laboratory
- Safety measures in a laboratory
- First aid in a laboratory
- Health precaution in a clinical laboratory
CLINICAL BIOCHEMISTRY
- Sample collection in a laboratory
- Equipment in a biochemical laboratory
- Sanitation in a biochemical laboratory
- Blood sugar test
- Renal function test
- Liver function test
- Lipid profile test
- Cardiac profiles
- Hormone profiles
- Enzyme profiles
- Electrolytes analysis
CLINICAL MICROBIOLOGY
- Sterilization and infection control
- Equipments used in a microbiological laboratory
- Growth requirements of a bacteria
- Bacterial culture
- Staining of bacteria, Different procedures.
- Antibiotic sensitivity test
- Serological tests
- Agglutination reactions
- Urine routine and microscopic examination
- Study of other body fluids including stool
- Virology
- Mycology
- Study of ova and parasites.
- Immunological reaction.
- Media
HEMATOLOGY AND BLOOD BANKING
- Estimation of hemoglobin
- ESR
- PVC
- RBC Count
- WBC Count
- Neubaurs chamber
- Platelet Count
- CT/BT/PTCC
- GBP
- Thick smear and thin smear
- Automated cell counting
- Blood Group
- Collection and Preservation of samples
- Labeling and transportation
- Blood transfusion
- Coombs Test
- Blood donor
- Blood recipient
- Complications of transfusion
- Quality control and blood bank safety
- Proficiency control of blood bank personnels
- Minor cross matching
- Major cross matching
HISTOTECHNOLOGY
- Introduction
- FNAC
- PAP Smear
- Cytology
- Cell structure
- Meiosis and mitosis
- Embryology
- Preparation of specimen
- Tissue processing (practical)
- H&E staining
PATHOLOGICAL INVESTIGATIONS 2
- Tests to distinguish between aerobic and anaerobic breakdown of carbohydrates
- Fermentation reactions
- Ability to use a specific substrate
- Testy for specific breakdown of products and aminoacids
- Tests for production of enzymes
DMLT & ONE YEAR POST DIPLOMA SYLLABUS
And any one option
1) Blood Bank Certificate
2) Transfusion Medicine
3) Clinical Microbiology
4) Hematology
5) Histotechnology Techniques
6) Clinical Biochemistry
7) Enzymology
Certificate for computer fundamentals .
Certificate for first –aid and emergency .
Certificate in medical ethics .
1. Blood Bank
Paper 1 – Immunohematology
Paper2 – Storage and Preservation of Blood
Paper3 – Transfusion Therapy
Paper 4 – Blood Components
Paper 5 – Practical + 6 month Internship in a registered blood bank
2. Transfusion Medicine
Paper 1 – Blood donor & Collection of Blood
Paper 2 – Transfusion reaction & Complication
Paper 3 – Screening for Diseases Transmitted Through Blood
Paper 4 – Transfusion Therapy
Paper 5 – Practical + 6 month Internship in a registered blood bank
3. Clinical Microbiology
Paper 1 – Sterilization & Disinfection
Paper 2 – Preparation of Stain & Reagents
Paper 3 – Preparation of Quality Control & Culture Media
Paper 4 – Colony Morphology, Staining Character and Identification
Paper 5 – Practical + 6 month Internship in a registered Diagnostic Centre
4. Hematology
Paper 1 – Blood & Anticoagulant
Paper 2 – Morphology Abnormal & Normal RBC's
Paper 3 – Blood Coagulation
Paper 4 – Hemorrhagic Disorders
Paper 5 – Practical + 6 month Internship in a registered Diagnostic Centre
5. Histotechnology Techniques
Paper 1 – Fixative , Processing & Impregnation
Paper 2 – Types of Microtome & Sectioning
Paper 3 – Special stain
Paper 4 – Museum Technique
Paper 5 – Practical + 6 month Internship in a registered Diagnostic Centre
6. Clinical Biochemistry
Paper 1 – Hormones
Paper 2 – Organs Function Test
Paper 3 – Water, Electrolyte & Acid base Balance
Paper 4 – Tissue Proteins & Body Fluids
Paper 5 – Practical + 6 month Internship in a registered Diagnostic Centre
7. Enzymology
Paper 1 – Nomenclature & Classification
Paper 2 – Chemical Nature of Enzymes
Paper 3 – Mechanism of Enzymes Action
Paper 4 – Enzyme Pattern in Diseases
Paper 5 – Practical + 6 month Internship in a registered Diagnostic Centre
DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY
FIRST YEAR PAPER MARKS:
| 1 | ENGLISH & COMPUTER FUNDAMENTAL | 100 |
| 2 | ANATOMY & PHYSIOLOGY | 100 |
| 3 | BIOCHEMISTRY | 100 |
| 4 | MICROBIOLOGY AND PARASITOLOGY | 100 |
| 5 | HEMATOLOGY & BLOOD BANKING -I | 100 |
| 6 | HISTOTECHNOLOGY -I | 100 |
| Total Theory | 600 | |
| Practical | 200 | |
| Internal Assessment | 200 | |
| GRAND TOTAL | 1000 |
SECOND YEAR PAPER MARKS:
| 1 | LABORATORY MANAGEMENT | 100 |
| 2 | CLINICAL BIOCHEMISTRY | 100 |
| 3 | CLINICAL MICROBIOLOGY | 100 |
| 4 | PATHOLOGICAL INVESTIGATION | 100 |
| 5 | HEMATOLOGY AND BLOOD BANKIING- II | 100 |
| 6 | HISTOTECHNOLOGY –II | 100 |
| Total Theory | 600 | |
| Practical | 200 | |
| Internal Assessment | 200 | |
| GRAND TOTAL | 1000 |
One year Post Diploma Paper Marks
| 1 | Paper | 100 |
| 2 | Paper | 100 |
| 3 | Paper | 100 |
| 4 | Paper | 100 |
| 5 | Paper | 200 |
| GRAND TOTAL | 600 |
| 1 | 1st Year Marks TOTAL | 1000 |
|---|---|---|
| 2 | 2nd Year Marks TOTAL | 1000 |
| 3 | One year Post Diploma Marks | 600 |
| GRAND TOTAL | 2600 |